KASP SNP Genotyping Service
High throughput Low cost High accuracy
KASP, or Kompetitive Allele Specific PCR, can be detected in a wide range of genomic DNA samples (even some DNA samples of complex genomes), Accurate biallelic determination of SNPs and InDels at specific sites.
What is SNP?
SNP, or single nucleotide polymorphism, refers to a DNA (deoxyribonucleic acid) sequence polymorphism caused by the variation of a single nucleotide at the genome level.SNP research has extensive significance: in the field of agriculture, it can be used for fine mapping of character genes, molecular assisted breeding, seed resource identification, etc. In the field of medicine, it is mainly the study of molecular genetic mechanism of diseases, disease gene localization, drug sensitivity or disease susceptibility site screening.
What does SNP research cover?
1. SNP research is mainly divided into SNP discovery and SNP genotyping.
2. The discovery of SNPs is the basis of application, and a small number of SNPS that may be associated with diseases or traits should be obtained through statistical analysis within the whole genome of a certain number of samples. At this point, the technology of gene chip or NGS (Next Generation Sequencing) has an advantage in detecting a large number of SNPS in a single sample.
SNP genotyping is an applied technique. This process requires the detection of a small number of SNPs in a large number of samples, and the gene chip or NGS technology is not suitable for this stage of research due to its high cost. KASP (Kompetitive Allele Specific PCR) plays an important role in SNP typing research as one of the mainstream SNP detection methods in the world, replacing traditional high-throughput sequencing due to its economical and flexible characteristics.
KassaSemagn et al. (2013) Single nucleotide polymorphism genotyping using Kompetitive Allele Specifi c PCR (KaSp): overview of the technology and its application in crop improvement. Molecular Breeding.